This digression leads back to Elijah’s highly explosive publication, which can only be found in the German-language alternative media in a marginal note in NDV News from February 15, 2023 [27]. It’s almost unnecessary to mention that the entire mainstream shrouds the research results in a cloak of silence and even the gaslighting fact-twisters haven’t touched the hot potato yet. The crucial aspect concerns an obvious graphical manipulation of the Western blot evaluations using image processing software by BioNTech. Authentic Western blots are characterized by highly specific substance-typical band patterns, which produce distortion effects at the edges of the blots due to the physical properties of the carrier materials and process technology, giving them an unmistakable arc shape. The following illustration (Fig.1) shows an example of a classic Western blot band pattern.
Each individual Western blot column corresponds to the separation of a specific substance into its respective characteristic components, which, depending on their size and charge, leave an always reproducible band pattern, similar to a fingerprint. For unknown substances, a calibration standard is carried along with a marker substance of known composition, which enables a quantitative assignment of the fractions in the samples to be analyzed (the information is given in the atomic mass unit kDa = kilodalton, in Fig. 1 this corresponds to the numbers on the ordinate on the left ). Blotting techniques are most commonly used in the molecular biological analysis of biogenic macromolecules (DNA, RNA, proteins, etc.). The invention goes back to Edwin Southern in 1975, who developed the method for DNA identification. Based on the direction of his last name, the modified variants for RNA analysis with Northern Blot and for protein detection were named Western Blot. In the first process step, the protein mixtures are separated on a gel substrate in an electric field (e.g. SDS-Page gel electrophoresis) and then transferred from the gel to another carrier membrane (nitrocellulose or PVDF) (either by renewed electric field induction or adsorbing capillary diffusion). The protein fragments can then be precisely identified on the carrier membrane using further detection agents, for example by chemiluminescence antibodies in immunoblots or by radioactive labeling in autoradiograms. The complexity of this time-consuming analysis technique also explains the formation of the typical distorted band patterns. BioNTech achieves the feat of producing Western blot graphics that show, in some cases, completely exact square-accurate plot profiles with identical pixel distribution and color intensity for two different proteins, in four different active ingredient batches, with six different sample concentrations [29]. It is precisely this completely impossible absurdity that laboratory practitioners came across when reviewing the documents submitted to the EMA and FDA by Pfizer/BioNTech. The relevant band patterns are illustrated in Fig. 2 and analyzed using the image processing program ImageJ [30].
In the upper part of Fig. 2 (area “A”) you can see the Western blot band patterns from the series of tests that were allegedly carried out and submitted to the regulatory authorities. Below are the associated ImageJ plot profile analyzes (orange and blue box assignment), with the evaluations of the peak grayscale intensities and pixel distributions. The repeating identical peak patterns (A – F and their combinations, e.g. GE1E2) in completely different sample mixtures are clearly visible, which is completely impossible [1(b)].
